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e ab f1136f rrid ab 3740202  (Elabscience Biotechnology)


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    Elabscience Biotechnology e ab f1136f rrid ab 3740202
    E Ab F1136f Rrid Ab 3740202, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/percp+anti+mouse+cd45+antibody/pmc13053763-27-8-5?v=Elabscience+Biotechnology
    Average 91 stars, based on 2 article reviews
    e ab f1136f rrid ab 3740202 - by Bioz Stars, 2026-07
    91/100 stars

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    (A). Flow cytometry workflow. The total leukocyte population was gated away from debris. (B) Single cells were then gated away from any multiplets. (C) Viable cells expressing low levels of CD45 were gated away from other cells and infiltrating macrophages. (D) Lastly, cells expressing low levels of CD45 and positive for CD11b were selected for the analysis, as they are microglia. (E) Microglia as a percentage of the total single cells in the sample graphed to highlight the comparison between brain and spinal cord. Data represents mean and error bars represent standard error of the mean. Statistical analysis was performed using a 3-way ANOVA with Tukey’s post-hoc analysis, n = 7 – 10 where each sample represents a different animal, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p <0.0001.

    Journal: bioRxiv

    Article Title: Spinal Cord Microglia Exhibit Impaired Repair Responses to Myelin Damage

    doi: 10.64898/2026.02.23.707565

    Figure Lengend Snippet: (A). Flow cytometry workflow. The total leukocyte population was gated away from debris. (B) Single cells were then gated away from any multiplets. (C) Viable cells expressing low levels of CD45 were gated away from other cells and infiltrating macrophages. (D) Lastly, cells expressing low levels of CD45 and positive for CD11b were selected for the analysis, as they are microglia. (E) Microglia as a percentage of the total single cells in the sample graphed to highlight the comparison between brain and spinal cord. Data represents mean and error bars represent standard error of the mean. Statistical analysis was performed using a 3-way ANOVA with Tukey’s post-hoc analysis, n = 7 – 10 where each sample represents a different animal, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p <0.0001.

    Article Snippet: The resulting cells were incubated in an FC block solution (Tonbo 70-0161, 1:100) for 10 minutes and then stained in an antibody mix for 30 minutes which consisted of CD45 violetFluor 450 (Tonbo 75-0451, 1:75), CD11b PerCP-Cyanine5.5 (Tonbo 65-0112, 1:75), Brilliant Violet 605 anti-mouse CD16 (Biolegend 158027, 1:75), FITC anti-mouse CD68 (Biolegend 137006, 1:75), APC/Fire 810 anti-mouse CX3CR1 (Biolegend 149054, 1:75), APC anti-mouse F4/80 (Biolegend 123116, 1:75), APC/Fire 750 anti-mouse CD73 (Biolegend 127221, 1:75), PE anti-mouse TREM2 (R&D Systems FAB17291P, 1:75), and viability dye (Tonbo 13-0871, 1:1000).

    Techniques: Flow Cytometry, Expressing, Comparison